Journal: PeerJ

Article Title: Exosomes in cancer: small vesicular transporters for cancer progression and metastasis, biomarkers in cancer therapeutics

doi: 10.7717/peerj.4763

Figure Lengend Snippet: Overview of the role of exosomes in multiple kinds of cancer.

Article Snippet: ACTB, TUBA1A, FN1, FNLA, CD61, HLA-A, LGALS3BP, Alix, RAB5B, RAB5C, SDCBP, VPF37B, CLTC, ARF1, ANXA2, ANXA5, HSC70, HSP72, RAC1, STOM, MFGE8, MVP, GNA12, PTGFRN, HBA1, tumor susceptibility gene-101 (TSG-101), and Grp94 , The inhoused established human HCC cell lines HKCI-C3 and HKCI-8 cells The hepatocellular carcinoma (HCC) cell line MHCC97L cells The immortalized hepatocyte cell line MIHA cells , qRT-PCR analysis, Ion Torrent Next-Generation Sequencing, and Western blotting , The internalization of exosomes could activate PI3K/AKT and MAPK signaling pathway, and promote secretion of MMP-2 and MMP-9 that favored cell invasion. Also, by proteome analysis Syndecan–syntenin–ALIX is known to support biogenesis of exosomes and the segregation of signaling cargo to these vesicles. The research also detected the components of endosomal protein sorting complex, such as VPS28 and VPS37, whose functions are required for exosome cargo sorting Process. , .

Techniques: Western Blot, Immunofluorescence, AChE Assay, Immunocytochemistry, Inhibition, Chromatin Immunoprecipitation, Marker, Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay, Derivative Assay, Biomarker Assay, In Vitro, In Silico, Diagnostic Assay, Isolation, Flow Cytometry, Variant Assay, In Vivo, Sampling, Modification, Transgenic Assay, Migration, Luciferase, Plasmid Preparation, In Vivo Imaging, Purification, Ex Vivo, Activation Assay, Injection, Endothelial Tube Formation Assay, Viability Assay, Matrigel Assay, Immunoprecipitation, Microscopy, Functional Assay, Knock-Out, Mass Spectrometry, Dot Blot, Electron Microscopy, Methylation, Chromatography, Over Expression, Mutagenesis, Microarray, Binding Assay, Transferring, Real-time Polymerase Chain Reaction, Bradford Assay, Transformation Assay, Animal Model, MTT Assay, Fluorescence, FACS, Diffusion-based Assay, Next-Generation Sequencing, Transplantation Assay, Sequencing, Histone Deacetylase Assay, Infection, Incubation, Transmission Assay, Blocking Assay, Luminex, Fractionation, Irradiation, Reporter Assay